Effects of Shensong-Yangxin Capsule on the Four Subtypes of CYP450 in Rats

doi:10. 3969 / j. issn. 2096-2266. 2021. 02. 003

Abstract

Abstract: Objective: To study the effects of Shensong-yangxin capsule on four subtypes of CYP450（CYP3A1, CYP2D1, CYP2C11
and CYP1A2）in rats by using the method of "Cocktail" probe drugs. Methods: Low, middle and high concentrations of Shensongyangxin
capsule were orally administered to rats for 15 days, respectively. On the 16th day, rats in each group were intraperitoneally
injected with "Cocktail" probe drugs （midazolam, metoprolol, omeprazole and caffeine）, and plasma concentrations and
pharmacokinetic parameters of each probe were determined. Results: Compared with the control group, low, middle and high
concentrations of Shengsong-yangxin capsule had significant effects on the pharmacokinetic parameters of omeprazole, metoprolol and
midazolam decreased, but no significant influence in caffeine. Conclusion: Shensong-yangxin capsule has no obvious effects on the
activity of CYP1A2, and it may have inhibitory effects on the activities of CYP2C11, CYP2D1, and CYP3A1.

Key words: Shensong-yangxin capsule, "Cocktail" probe drugs, cytochrome P450

CLC Number:

R285.5

Miao Zhimin, Lai Yong. Effects of Shensong-Yangxin Capsule on the Four Subtypes of CYP450 in Rats[J]. Journal of Dali University, 2021, 6(2): 15-19.

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References

〔1〕胡金，徐惠波. 中药抗心律失常作用研究进展〔J〕. 中国
医药导报，2011，8（32）：9-11.
〔2〕代丽. 参松养心胶囊联合美托洛尔治疗心律失常的效果
观察〔J〕. 临床研究，2019，27（2）：116-118.
〔3〕王银环. 参松养心胶囊联合辛伐他汀治疗心律失常的临
床疗效及对心电图和血脂的影响〔J〕. 光明中医，2019，
34（1）：120-122.
〔4〕黄山英. 参松养心胶囊联合曲美他嗪对冠心病合并心律
失常的疗效〔J〕. 中华心脏与心律电子杂志，2019，7（2）：
86-88.
〔5〕王用，史云桃，王文彬. 胺碘酮联合参松养心胶囊治疗阵
发性房颤疗效〔J〕. 中西医结合心血管病电子杂志，
2019，7（21）：165.
〔6〕梁莹，何飞，刘源. 华法林联合参松养心胶囊对心房颤动
的疗效及对患者血清hs-CRP 水平的影响〔J〕. 海峡药
学，2019，31（3）：138-139.
〔7〕骆玉华. 参松养心胶囊联合富马酸比索洛尔治疗室性
早搏疗效分析〔J〕. 中国冶金工业医学杂志，2019，36
（1）：64.
〔8〕梁莹，何飞，张金盈. 参松养心胶囊联合瑞替普酶治疗急
性ST段抬高型心肌梗死的临床研究〔J〕. 现代药物与临
床，2019，34（6）：1635-1640.
〔9〕杨娇，赖泳. 细胞色素P450基因多态性与肿瘤发生关联
性的研究进展〔J〕. 广东医学，2017，38（21）：3363-3366.
〔10〕WANG H，TOMPKINS L M. CYP2B6：New Insights into
a Historically Overlooked Cytochrome P450 Isozyme〔J〕.

Curr Drug Metab，2008，9（7）：598-610.

〔11〕魏伟，吴希美，李元建. 药理实验方法学〔M〕. 4 版.
北京：人民卫生出版社，2010.
〔12〕BREIMER D D，SCHELLENS J H. A "Cocktail" Strategy
to Assess in vivo Oxidative Drug Metabolism in Humans〔J〕.
Trends Pharmacol Sci，1990，11（6）：223-225.
〔13〕GOH B C，REDDY N J，DANDAMUDI U B，et al. An
Evaluation of the Drug Interaction Potential of Pazopanib，
an Oral Vascular Endothelial Growth Factor Receptor
Tyrosine Kinase Inhibitor，Using a Modified Cooperstown
5+1 Cocktail in Patients with Advanced Solid Tumors〔J〕.
Clin Pharmacol Ther，2010，88（5）：652-659.
〔14〕MORI K，HASHIMOTO H，TAKATSU H，et al. Cocktail-
Substrate Assay System for Mechanism-Based Inhibition
of CYP2C9，CYP2D6，and CYP3A Using Human Liver
Microsomes at an Early Stage of Drug Development〔J〕.
Xenobiotica，2009，39（8）：415-422.
〔15〕BOGAARDS J J，BERTRAND M，JACKSON P，et al. De⁃
termining the Best Animal Model for Human Cytochrome
P450 Activities：A Comparison of Mouse，Rat，Rabbit，
Dog，Micropig，Monkey and Man〔J〕. Xenobiotica，2000，
30（12）：1131-1152.
〔16〕MAGGO S D S，SAVAGE R L，KENNEDY M A. Impact of
New Genomic Technologies on Understanding Adverse
Drug Reactions〔J〕. Clin Pharmacokinet，2016，55（4）：
419-436.
〔17〕莫延红，牛璇，张兆辉. CYP2C19基因多态性对临床
常见药物代谢的影响〔J〕. 卒中与神经疾病，2019，26
（4）：499-507.
〔18〕魏晓冬，张峰，李娟，等. CYP3A4 基础与临床研究进
展〔J〕. 现代生物医学进展，2014，14（11）：2180-2182.

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[2]	CHEN Lei, CUI Chu-Jun, WANG Min, JIANG Bei. Influence of the Extracts from Three Astragalus Plants on Platelet Aggregation [J]. , 2019, 4(10): 22-26.
[3]	XU Ya, DIAO Qi. Study on the Mechanism of Antihypertensive Effect of Plantain Seed Based on Network Pharmacology [J]. , 2019, 4(8): 33-38.
[4]	HU Zan, LI Cai-Gong- , LI Yin-Juan, CHEN Dun-Ya- , LA Hai, GENG Ling. Effects of Geranium strictipes on Gastroparesis Model in Mice and Gastric Smooth Muscle Strips in Rats [J]. , 2019, 4(4): 7-11.
[5]	YANG Qian, SHU Sai-Ying, DAN Chao-Fang, DAN Cheng-Yu, LA Hai. Effects of Ethanol Extracts from Different Parts of Taraxacum mongolicum Whole Herb on Cariogenic Bacteria [J]. , 2019, 4(4): 18-21.
[6]	DAN Cheng-Yu, DAN Chao-Fang, YANG Qian, SHU Sai-Ying, LA Hai. Effect of the Extract of Taraxacum mongolicum on Remineralization of Bovine Incisor Root Caries [J]. , 2019, 4(4): 22-25.
[7]	CHEN Jin-Ru, LIU Xi, LI Dong-Mei, DING Yun, LIU Guang-Meng, LI Chun-Yan. Study on the Anti-hepatic Fibrosis Effects of Pinecone Ethanol Sediments from Pinus yunnanenis Fr. in vitro [J]. , 2018, 3(8): 6-11.
[8]	Lu Lei, Feng Chao, Cui Xuebing, Yang Jiwu, Qin Yan, Liu Rengui. Protective Effect of Grape Seed Proanthocyanidin Extract on Lower Extremity Arteriosclerosis Obliterans [J]. , 2017, 2(4): 36-40.
[9]	Li Jiao, Zhang Bingqing, Liu Guangming, Xia Mengkai, Zhang Xiaodong, Liu Xiaobo. Effect of Periplaneta americana de-fat Extract on Immune Function in Mice [J]. J4, 2015, 14(10): 1-5.
[10]	Liu Chengjiao, Xia Mengkai, Li Yang, Wang Ying. In Vitro Anti-tumor Effects of Extracts from Juglans sigillata Shell [J]. J4, 2015, 14(8): 15-17.
[11]	Yang Lin, Wang Tao, Lan Hai. Antibacterial Activities of the Ethyl Acetate Part and the Water Part of Geranium Trictipes R. Kunth to Actinomyces Viscosus in Vitro [J]. J4, 2015, 14(4): 8-11.
[12]	Li Qiaoyun, Yu Zhengyong, Wang Na, Li Wanyu, Du Yi Min, Liu Xiaobo. Experimental Investigation into the Effects of Xigui on the Function of Monocyte of sleep-deprived Mice [J]. J4, 2015, 14(4): 17-20.
[13]	GUO Mei-xian, YANG Yi-wen, LIU Xiao-bo, SHI Gui-rong. Protective Effect of Rubus delavayi on Alcoholic Hepatitis in Rats [J]. J4, 2014, 13(10): 1-4.
[14]	LI Daihang, ZHU Wanyun, DUAN Suying, LAI Yong. Fuzz Myrobalan Bark Alcohol Extract Determination of Median Lethal Dose and Its Effect on Intestinal Muscle [J]. J4, 2014, 13(10): 10-13.
[15]	SHI Zhi-Ting, LIU Xi, ZHANG Hai-Zhu. Experimental Study on Acute Toxicity of Anti-HIV-1 Active Extracts from Pinecone of Pinus yunnanensis [J]. J4, 2014, 13(8): 4-6.